Chemotherapy, a therapeutic modality utilizing highly toxic drugs to kill cancer cells, has played a pivotal role in cancer treatment over the past decades.[1] However, poor drug delivery, unsatisfactory antitumor performance, severe side effects, and drug resistance greatly impair their clinical outcome.[2] Benefit from nanotechnology, effective delivery of chemotherapeutic drugs is achieved by the enhanced permeability and retention (EPR) effect, which prolongs their circulation time and enhances the tumor‐specific accumulation.[3] Among various nanomedicine systems, metal–organic framework (MOF) nanoparticles have unique advantages beyond drug loading and controlled delivery based on tailor‐designed porous structure.[1d,4] The presence of metal ions and organic ligands endows the resultant MOF platforms with excellent theranostic capability. For example, chemodynamic therapy (CDT) that catalytically converts intracellular hydrogen dioxide (H2O2) into highly toxic reactive oxygen species (ROS) through therapeutic reactions, especially Fenton‐like reactions, can be integrated into the MOF nanoparticles to promote the anticancer performance.[5] Various studies have demonstrated that the nanomedicines combining chemotherapy and CDT exhibit excellent synergy effect in suppressing tumor growth and inhibiting tumor metastasis.[5d,6]
Unfortunately, insufficient endogenous H2O2 in the tumor tissue significantly restricts the further applications of CDT.[7] The existed strategies such as directly encapsulating exogenous H2O2 are facing several serious issues, such as the inevitable damage to normal tissues caused by the leakage of H2O2 from carriers.[8] Glucose oxidase (GOx) with the ability to produce H2O2 by catalyzing glucose meets the requirement of H2O2 generation in situ. However, the uncontrollable reaction between GOx and glucose during delivery processes and relative low operational stability of GOx hamper the practical in vivo application of this strategy.[5d,9]
Therefore, developing nanoformulations that can specifically and efficiently catalyze glucose to H2O2 transforming will achieve the purpose of precisely killing cancer cells, together with MOF‐based nanomedicine. Fortunately, gold nanoparticles (Au NPs) with small size exhibit highly efficient and stable GOx‐mimic catalytic activity, which can oxidize intracellular glucose to produce H2O2.[10] More importantly, the catalytic activity of Au NPs can be rationally controlled by their surface hydrophobicity and subsequent interaction with water soluble glucose, enabling to specifically activate their enzyme‐mimic catalytic capability in cancer cells, thus solving tricky issues faced by the natural GOx for cancer treatments.
Herein, we prepare a sophisticated nanomedicine with cascade catalytic capability for cancer chemo/chemodynamic therapy on the basis of a hybridization strategy (Scheme 1). Using iron(III) meso‐tetra(4‐carboxyphenyl)porphine chloride TCPP(Fe) and zirconyl cluster as building blocks, MOF nanoparticles (FeMOF NPs) are fabricated that are further utilized as platforms to construct the hybrid composite by in situ growth of small Au NPs on the surface. FeMOF NPs with porous structure can be used to encapsulate a hydrophobic chemotherapeutic drug (camptothecin, CPT) and the anchored Au NPs can be further modified by 1‐dodecanethiol (C12SH) and methoxy polyethylene glycol thiol (PEG‐SH) to improve the stability of the nanomedicine and to block the catalysis during circulation. Interestingly, the nanohybrids completely collapse after cellular internalization triggered by the intracellular phosphate due to its stronger coordination with zirconium, thus boosting the drug release and activating the cascade catalytic reactions. Au NPs act as artificial enzyme to oxidize the intracellular glucose to elevate the endogenous H2O2 level, which is the chemical fuel for Fenton reaction catalyzed by TCPP(Fe) to produce highly active OH∙. It should be emphasized that the consumption of glucose also contributes to the anticancer efficacy through a starvation strategy. Attributing to smart nanotechnology and the EPR effect, the circulation time of PEG‐Au/FeMOF@CPT NPs is remarkably prolonged and its tumor accumulation is greatly enhanced, facilitating to improve therapeutic efficacy and avoid systemic toxicity. In vitro and in vivo studies verify the satisfactory therapeutic performance of this hybrid nanomedicine, significantly inhibiting the tumor growth combining chemotherapy and chemodynamic therapy.
FeMOF NPs were first prepared through a solvothermal method in dimethylformamide at 95 °C for 5 h. Monodispersed nanoparticles with the diameter around 50 nm were observed in transmission electron microscopy (TEM) image (Figure 1a and Figure S1, Supporting Information). Au/FeMOF NPs were further fabricated using FeMOF NPs as platforms by directly reducing HAuCl4 in water using sodium borohydride as a reductant. As shown in Figure 1b,d, dark spots corresponding to the Au NPs ≈5 nm in diameter were observed on the exterior surface of Au/FeMOF NPs. Zeta potential of FeMOF NPs decreased from 33.1 to −46.5 mV upon formation of Au/FeMOF NPs (Figure S2, Supporting Information). Compared with FeMOF NPs, the absorption of Au/FeMOF NPs in the range of 500–700 nm remarkably increased (Figure 1f). The reason was that the distance between the Au NPs was close enough, thus resulting in the plasmonic coupling among the particles. Brunauer–Emmett–Teller surface area characterization demonstrated that the crystallinity of MOF scaffold in the hybrid composite was effectively maintained after the formation of Au/FeMOF NPs with a surface area as high as 1451 m2 g−1 (Figure S3, Supporting Information). Additionally, the pore size distribution was measured to be 1.7 nm, confirming that the MOF NPs were able to load anticancer drugs (Figure S4, Supporting Information). Indeed, CPT, a topoisomerase I inhibitor, could be successfully encapsulated into the porous Au/FeMOF nanoparticles with a loading content of 7.7% mainly driven by the π–π stacking interaction between CPT and TCPP(Fe) and the coordination between Zr and the quinine group of CPT. UV‐vis spectrum confirmed the co‐existence of the specific absorption corresponding to Au/FeMOF nanoparticles and CPT (Figure 1f). As evidenced by TEM and dynamic light scattering (DLS) measurements (Figures S5 and S6, Supporting Information), the drug encapsulation did not change the size and morphology of the hybrid nanoparticles.
In order to further improve the colloidal stability of Au/FeMOF NPs and block the catalytic capability of the anchored Au NPs in physiological environment, C12SH and PEG‐SH were employed to modify Au/FeMOF NPs via AuS bond. The hydrophobic alkyl chains from C12SH provided a protective shell to prevent the penetration of phosphate ion and glucose, potentially preserving the nanostructure and silencing the catalytic reaction. On the other hand, PEGylation of Au/FeMOF NPs facilitated to enhance their dispersion ability and avoid the absorption by the proteins in the bloodstream. TEM image confirmed that the morphology of the hybrid nanomaterials was maintained after this modification (Figure 1c,d). Energy dispersive spectroscopy (EDS) elemental mapping analyses confirmed the existence of each element (Au, S, Fe, and Zr) in the PEG‐Au/FeMOF NPs, providing a direct evidence for the successful fabrication of the hybrid nanomaterial (Figure 1e and Figure S7, Supporting Information). DLS measurements suggested a slight increase in the average diameter after this noncovalent modification (Figure 1g). It should be pointed out that Au/FeMOF NPs formed large aggregates in saline. This phenomenon was inhibited by the PEGylation (Figure 1g), indicating that the surface modification was favorable to enhance their dispersion in physiological solution. Zeta potential of the PEGylated Au/FeMOF NPs was determined to be −2.4 mV, while the neutral charge was favorable to prolong circulation time and enhance cellular endocytosis of NPs (Figure 1h and Figure S2, Supporting Information).[2i] More importantly, this surface modification significantly enhanced the stability of PEG‐Au/FeMOF NPs in phosphate buffered saline (PBS). As shown in TEM images, the MOF structures of FeMOF and Au/FeMOF completely collapsed after culturing in 2.00 × 10−3 m of PBS (Figure 2b,e). The fast release of porphyrin ligands shown in Figure 2k also provided convincing evidence that modification was required due to the stability of the FeMOF and Au/FeMOF. In contrast, the nanostructure of PEG‐Au/FeMOF NPs could be maintained for more than 1 day (Figure 2h,k). It should be noted that the drug loading contents for Au/FeMOF NPs and PEG‐Au/FeMOF NPs (7.1%) were comparable, suggesting that the decoration of Au NPs and PEGylation had negligible impact on drug encapsulation. Moreover, the resultant nanomedicine (PEG‐Au/FeMOF@CPT NPs) exhibited excellent dispersion in saline solution, PBS (2 × 10−3 m) containing 10% fetal bovine serum and culture medium for 48 h (Figure S8, Supporting Information), making them suitable for potential biomedical applications.
The phosphate‐responsiveness of PEG‐Au/FeMOF NPs was further studied in the solution containing much higher phosphate concentration (10.0 × 10−3 m). Figure 2k indicated that negligible release of TCPP(Fe) was detected in saline, and only little amount (12.2%) of TCPP(Fe) was leaked from PEG‐Au/FeMOF NPs in the presence of PBS (2.00 × 10−3 m) within 48 h. In contrast, 84.5% and 85.3% of TCPP(Fe) were released from the naked FeMOF NPs and Au/FeMOF NPs in PBS (2.00 × 10−3 m) after 24 h incubation, respectively. These results indicated that the hybridization of FeMOF NPs by Au NPs and subsequent surface modification by C12SH and PEG‐SH significantly improved the stability of PEG‐Au/FeMOF NPs. Interestingly, the release speed of TCPP(Fe) was significantly accelerated by incubating PEG‐Au/FeMOF NPs in PBS containing 10.0 × 10−3 m phosphate ions (Figure 2k). TEM image (Figure 2i) revealed that the nanoparticulate structure of PEG‐Au/FeMOF NPs was totally collapsed, and the detached Au NPs were well dispersed. The reason was that the coordination interactions between Zr and phosphate ions at high concentration degraded the MOF platform. Notably, phosphate‐sensitive release of CPT was also confirmed. The decreased solution pH protonated the carboxylate groups of TCPP(Fe) and quinine group of CPT, accelerating the dissociation of MOF platform and CPT release. The nanomedicine was stable in saline and PBS with low concentration of phosphate (2.00 × 10−3 m), while the release rate was dramatically increased in PBS containing 10.0 × 10−3 m of phosphate, confirming that the loaded drug could be completely released after cellular internalization by the cancer cells (Figure 3a).
In this hybrid nanomedicine, cascade reactions took place to use glucose as chemical fuel to produce highly toxic radicals. Au NPs can act as GOx‐like enzyme to oxidize glucose to produce H2O2 and gluconic acid.[10] The resultant H2O2 was further catalyzed by TCPP(Fe) to generate OH· through Fenton reaction. The generation of H2O2 and gluconic acid (reflected by pH) from the first oxidation reaction was dependent on the concentration of glucose. The reaction quickly reached an equilibrium within 6 h (Figures S9 and S10, Supporting Information), which confirmed the high catalytic efficiency of PEG‐Au/FeMOF NPs. In the second reaction, methylene blue (MB) was employed as an indicator, which was decomposed by the generated OH· accompanied with the disappearance of blue color. As shown in Figure 3b, no apparent changes in the MB absorbance at 500−700 nm were detected for MB solution incubated with PEG‐Au/FeMOF NPs alone. Upon addition of glucose, the degradation of MB by PEG‐Au/FeMOF NPs increased along with enhanced phosphate concentration (Figure S11, Supporting Information). The reason was that the oxidation of glucose by PEG‐Au/FeMOF NPs under phosphate‐free environment was effectively blocked due to the surface modification, arising from the deactivation of catalytic ability and inhibition of the glucose penetration. Another possible reason was that the integrity of the MOF structure also diminished the Fenton reaction. Interestingly, the decomposition rate of MB was remarkably accelerated using the solution containing high phosphate concentration (10.0 × 10−3 m), which was a convincing evidence that the collapse of the hybrid activated the cascade catalytic reactions (Figure 2b and Figure S11, Supporting Information). It should be noted that negligible changes in MB absorption were monitored by replacing TCPP(Fe) by meso‐tetra(4‐carboxyphenyl)porphine chloride (HTCPP) during the preparation of PEG‐Au/HMOF NPs, emphasizing that TCPP(Fe) acted as the catalyst in the hybrid NPs. Compared with free FeCl3 with an extremely high reactivity, a sustained decomposition of MB was observed (Figure S11, Supporting Information). This property was extremely important in biomedical application that could inhibit the catalytic reactions during circulation, thus avoiding side effect toward normal tissues. The phosphate level in extracellular/plasma fluid is about 2.00 × 10−3 m, while this number can reach as high as 10.0 × 10−3 m inside cells.[11] Considering the difference in extracellular and intracellular phosphate concentrations, this nanomedicine guaranteed its safety during delivery process, while efficiently released the loaded drug and activated the cascade catalytic capability after cellular uptake by cancer cells.
With the nanomedicine in hand, the cellular internalization was studied by tracing the intrinsic red fluorescence of TCPP(Fe) using HepG2 cells, a human liver cancer cell line. Incubating the cells with PEG‐Au/FeMOF@CPT NPs for 8 h resulted in the appearance of strong red fluorescence in cytoplasm (Figure 3c). Confocal laser scanning microscopy (CLSM) image also showed that the blue fluorescence derived from CPT homogenously diffused in entire cells. Quite a large portion of CPT had penetrated into the nucleus as evidenced by the overlap of the blue fluorescence and green fluorescence from the nucleus stained by Syto‐9 dye. Additionally, the time‐dependent endocytosis of PEG‐Au/FeMOF@CPT NPs by HepG2 cells was further tracked by detecting the intracellular Au and Zr amount using inductively coupled plasma mass spectrometry (ICP‐MS). Figure S12 in the Supporting Information confirms the co‐existence of Au and Zr in HepG2 cells and the amount increased progressively by extending the culture time.
The endocytic pathways of PEG‐Au/FeMOF@CPT NPs were further investigated using ICP‐MS by pre‐treating HepG2 cells with different pharmaceutical inhibitors for endocytosis (Figure 3d). The cellular uptake of the nanomedicine was significantly inhibited at 4 °C, which meant that the endocytosis was energy‐dependent. A 38.6%, 17.9%, and 23.4% reduction in internalization was observed by pre‐treating the cells with chlorpromazine (CPZ), genistein (Gen), or amiloride‐HCl (AMD), respectively, indicating that the endocytosis was mainly through clathrin‐mediated endocytosis, caveolae‐mediated endocytosis, and macropinocytosis pathway.[12]
Before evaluating the anticancer efficacy, the feasibility of Fenton reaction in the cells was checked. 2,7‐Dichlorofluorescin diacetate (DCFH‐DA) was chosen as a fluorescent probe to detect the generation of ROS in HepG2 cells, whose green fluorescence was waked up after oxidization by OH·. CLSM images in Figure 3e indicated that minor changes in green fluorescence were found for the cells incubated with FeMOF NPs. The reason was that the endogenous H2O2 was insufficient for Fenton reaction to generate a highly reactive OH·. About 6.74‐fold enhancement of ROS was achieved by treating the cells with PEG‐Au/FeMOF NPs (Figure 3f), validating the ROS level was significantly elevated through the oxidation of glucose followed by a Fenton reaction. The intracellular production of ROS was greatly inhibited by removing the iron catalyst using PEG‐Au/HMOF NPs as indicated by the low green fluorescence in CLSM images, further verifying the cascade catalysis involving the generation of OH·.
3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was adopted to evaluate the anticancer efficacy of the combination chemo/chemodynamic therapy against HepG2 cells (Figure 3g). In the case of FeMOF NPs, negligible cytotoxicity was recorded even when the concentration was higher than 50 µg mL−1. For chemotherapy alone, the half‐maximal inhibitory concentration (IC50) of CPT was calculated to be 206 ± 22 × 10−9 m. The cascade starvation and chemodynamic therapy (PEG‐Au/FeMOF NPs) effectively inhibited the cell proliferation with an IC50 value of 3.51 ± 0.26 µg mL−1. Excitingly, the cells treated with chemotherapy and chemodynamic therapy (PEG‐Au/FeMOF@CPT NPs) resulted in the lowest IC50 value (0.31 ± 0.04 µg mL−1). Notably, the IC50 value of PEG‐Au/HMOF@CPT NPs was much higher than that of PEG‐Au/FeMOF@CPT NPs, amplifying that the chemodynamic effect of this hybrid nanomedicine played a pivotal role in its anticancer behavior. According to the MTT data, the combination index was calculated to be 0.41, which confirmed that the combination therapy had an excellent synergy. Moreover, the synergetic anticancer efficacy of PEG‐Au/FeMOF@CPT NPs was studied using Annexin‐V/PI dual‐staining. By quantitatively distinguishing the cells in different apoptotic/necrotic status, the chemo/chemodynamic therapy resulted in a large portion of apoptosis (48.7%) and necrosis (14.6%), further confirming the excellent anticancer efficacy of PEG‐Au/FeMOF@CPT NPs.
Prior to the antitumor studies, the in vivo safety and pharmacokinetic behaviors of the nanomedicine should be carefully evaluated. As shown in Figure 3i, treatment of PEG‐Au/FeMOF@CPT NPs for different time did not cause apparent hemolysis in the test concentration range. The great blood compatibility shall be attributed to the PEGylation of the nanomedicine and subsequently avoid the damage of the membrane of the red blood cells.[13]
The circulation half‐life of PEG‐Au/FeMOF@CPT NPs was determined using ICP‐MS by measuring the Zr amount in blood different time post intravenously (i.v.) injection (Figure S13, Supporting Information). Different from free CPT that was rapidly cleared from the blood with a circulation half‐life of 0.35 ± 0.05 h, the circulation half‐life of PEG‐Au/FeMOF@CPT NPs was calculated to be 4.27 ± 0.36 h, which was about 12.2‐fold of free CPT. Additionally, PEG‐Au/FeMOF@CPT NPs had much larger total area under the curve than that of CPT, further confirming that the circulation time of PEG‐Au/FeMOF@CPT NPs was significantly improved by taking advantage of nanotechnology. More importantly, the Au/Zr ratio in blood at different time post injection almost kept constant (Figure S14, Supporting Information), this meant that the nanomedicine was stable during circulation mainly attributing to the hybridization and surface modification. This property was extremely important to specifically activate the anticancer efficacy in cancer cells while reduce the systemic toxicity of this nanomedicine.
The PEG chains on the surface of PEG‐Au/FeMOF@CPT NPs can form a “brush‐like” shell that greatly prevents proteins adsorption, which facilitated to prolong the circulation time and thus promote the accumulation of PEG‐Au/FeMOF@CPT NPs in tumor tissue.[2i] Using ICP‐MS, the time‐dependent biodistribution of PEG‐Au/FeMOF@CPT NPs in the main organs and tumors was quantitatively measured. As shown in Figure 4a, PEG‐Au/FeMOF@CPT NPs were highly accumulated in the tumors attributing to the extended circulation time and EPR effect. The intratumoral amount gradually increased from 5.87 ± 0.62 to 7.87 ± 0.68%ID g−1 at 12 and 24 h post i.v. injection, and the tumor accumulation of PEG‐Au/FeMOF@CPT NPs maintained as high as 6.31 ± 0.57%ID g−1 even at 48 h post injection. In sharp comparison, the tumor accumulation of free CPT was quite low and most of the toxic drug stuck in lung (Figure 4b), because the hydrophobic CPT was formed into large aggregations in blood after injection. These differences demonstrated the advantages of nanoformulations in enhancing anticancer efficacy while reducing the side effects of free drug. In vivo fluorescent imaging was also employed to directly visualize the tumor accumulation of PEG‐Au/FeMOF@CPT NPs by using the intrinsic fluorescence of the porphyrin ligands (Figure 4c). 24 h post injection, strong signal was observed in tumor tissue, in good agreement with the result obtained from ICP‐MS investigations. CLSM image of the tumor tissue from the mice formulated with PEG‐Au/FeMOF@CPT NPs at 24 h post injection further demonstrated that the nanomedicine could deeply penetrate into the tumor, which was important to effectively kill the cancer cells (Figure S15, Supporting Information).
With the satisfactory synergistic anticancer performance and high tumor accumulation of PEG‐Au/FeMOF@CPT NPs in hand, in vivo antitumor studies were performed using HepG2 tumor‐bearing nude mice. When the tumor volume reached around 100 mm3, the mice were divided randomly into five groups and injected with PBS, CPT, PEG‐Au/FeMOF NPs, PEG‐Au/HMOF@CPT NPs, and PEG‐Au/FeMOF@CPT NPs, respectively. Compared with the mice treated with PBS, only moderate therapeutic result was observed for the ones treated with CPT and PEG‐Au/FeMOF NPs, mainly attributing to the unsatisfactory anticancer effect of single chemotherapy and CDT (Figure 4d). Administration with PEG‐Au/HMOF@CPT NPs resulted in relatively higher antitumor efficacy, because the EPR effect facilitated the high tumor accumulation of CPT. The consumption of glucose due to the oxidation mediated by Au NPs also played a role in starvation therapy. By taking advantages of the EPR effect, tumor‐specific drug release, and synergistic anticancer efficacy, the tumor growth was significantly suppressed for the mice treated with PEG‐Au/FeMOF@CPT NPs. According to the tumor volume, the inhibition ratio was determined to be 16.7%, 30.1%, 44.3%, 62.7%, and 85.6% for the mice treated with PEG‐Au/FeMOF NPs, CPT, PEG‐Au/HMOF@CPT NPs, and PEG‐Au/FeMOF@CPT NPs, respectively (Figure S16, Supporting Information). Benefiting from the excellent therapeutic performance, the median survival ratio of mice treated with PEG‐Au/FeMOF@CPT NPs was significantly extended (Figure 4e), which was much longer than the other formulations. Hematoxylin and eosin (H&E) staining indicated that the formulation of MOF‐Au‐PEG@CPT resulted in the highest level of apoptotic and necrotic cells in tumor tissues (Figure 4g). Transferase‐mediated dUTP nick end‐labeling (TUNEL) staining indicated a 67.1% cell apoptosis in tumor sites from the mice administrated with MOF‐Au‐PEG@CPT, which was the highest among all the groups (Figure 4f,h), demonstrating that the antitumor performance was greatly enhanced when combining chemotherapy and CDT together.
The systemic toxicity was assessed by detecting the changes in body weight of mice treated with these formulations. Figure S17 in the Supporting Information suggested that the administration of free CPT led to an obvious weight loss due to the nonspecific distribution of the toxic drug. The toxicity of CPT was greatly reduced by encapsulating the drug into the sophisticated nanomedicine. During the therapeutic period, no apparent body weight reduction was noticed from the mice received PEG‐Au/FeMOF@CPT NPs at the same dosage. Moreover, no evident symptoms of toxic effects were observed for the mice, such as drinking, eating, urination, grooming, activity, and neurological status. H&E staining of the liver and kidney tissues from the mice administrated with PEG‐Au/FeMOF@CPT NPs showed that no damage or inflammatory lesion was observed in these organs (Figures S18 and S19, Supporting Information). Blood biochemistry tests further provided evidences for the negligible long‐term potential toxicity of this nanomedicine. Compared with the healthy mice, no obvious changes in the levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), and blood urea nitrogen (BUN) for the mice injected with PEG‐Au/FeMOF@CPT NPs, while these parameters were greatly increased for the mice receiving free CPT treatment (Figures S20–S24, Supporting Information). These studies confirmed the low systemic toxicity of this smart nanomedicine benefiting from the nanotechnology.
In conclusion, a smart nanomedicine was prepared for cancer chemo/chemodynamic therapy based on a hybrid nanomaterial, in which Au NPs were grown in situ on the MOF NPs. The highly porous MOF NPs were employed to load CPT and the hybridization strategy effectively increased the stability of the resultant nanocomposites. Interestingly, the Au NPs were able to oxidize the intracellular glucose to produce H2O2, which was further used to generate highly toxic •OH for cancer chemodynamic therapy. Surface modification guaranteed the stability of this nanomedicine, and the cascade catalytic reactions were inhibited during circulation. Triggered by the high concentration of phosphate ions, the hybrid nanomaterials collapsed after cellular internalization, resulting in the burst release of the loaded drug and activation of the cascade reactions. Attributing to the EPR effect and sophisticated designs of this nanomedicine, the circulation time was greatly prolonged and the tumor accumulation was significantly improved, which were favorable to enhance its antitumor performance and reduce side effects. In vivo studies demonstrated the excellent synergy combining chemotherapy and chemodynamic therapy, effectively suppressing the tumor growth. This interesting work provided new strategies for the fabrication of theranostic hybrid nanomedicines to overcome limitations faced by the existed modalities, which hold promising potential in cancer treatment.
Y.D., H.X., C.X., and Z.T. contributed equally to this work. The financial support was from the National Natural Science Foundation of China (grant no. 81773096, 51822306), the National Key Research and Development Program of China (2016YFE0132700), Key Research and Development Program of Zhejiang Province (no. 2018C03085), the Public Welfare Technology Research Project of Zhejiang Province (no. LGD19C040006), the General Research Project of the Zhejiang Provincial Education Department (no. Y201840044), and the Fundamental Research Funds for the Central Universities of China (2019XZZX004‐07). All animal experiments were carried out in accordance with the Institute of Laboratory Animal Resources guidelines. Ethical approval (no. 2018–285) was granted by the Institutional Animal Care and Use Committee of Zhejiang Academy of Medical Sciences, China. HepG2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).
The authors declare no conflict of interest.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
© 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Abstract
The incorporation of new modalities into chemotherapy greatly enhances the anticancer efficacy combining the merits of each treatment, showing promising potentials in clinical translations. Herein, a hybrid nanomedicine (Au/FeMOF@CPT NPs) is fabricated using metal–organic framework (MOF) nanoparticles and gold nanoparticles (Au NPs) as building blocks for cancer chemo/chemodynamic therapy. MOF NPs are used as vehicles to encapsulate camptothecin (CPT), and the hybridization by Au NPs greatly improves the stability of the nanomedicine in a physiological environment. Triggered by the high concentration of phosphate inside the cancer cells, Au/FeMOF@CPT NPs effectively collapse after internalization, resulting in the complete drug release and activation of the cascade catalytic reactions. The intracellular glucose can be oxidized by Au NPs to produce hydrogen dioxide, which is further utilized as chemical fuel for the Fenton reaction, thus realizing the synergistic anticancer efficacy. Benefitting from the enhanced permeability and retention effect and sophisticated fabrications, the blood circulation time and tumor accumulation of Au/FeMOF@CPT NPs are significantly increased. In vivo results demonstrate that the combination of chemotherapy and chemodynamic therapy effectively suppresses the tumor growth, meantime the systemic toxicity of this nanomedicine is greatly avoided.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
; Wang, Weilin 1 1 Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Hangzhou, Zhejiang, China; Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang Province, Hangzhou, Zhejiang, China; Clinical Medicine Innovation Center of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Disease of Zhejiang University, Hangzhou, Zhejiang, China; Clinical Research Center of Hepatobiliary and Pancreatic Diseases of Zhejiang Province, Hangzhou, Zhejiang, China
2 MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, China; Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Hangzhou, Zhejiang, China