Neuroprotective Effect of Etomidate in the Central Nervous System of Streptozotocin-Induced Diabetic Rats

Neurochem Res (2006) 31:777783DOI 10.1007/s11064-006-9076-0ORIGINAL PAPERNeuroprotective Effect of Etomidate in the Central Nervous

System of Streptozotocin-Induced Diabetic RatsOzkan Ates Neslihan Yucel Suleyman R. Cayli Eyup Altinoz Saim Yologlu Ayhan Kocak Celal Ozbek Cakir Yusuf TurkozAccepted: 18 April 2006 / Published online: 21 June 2006

Springer Science+Business Media, Inc. 2006Abstract It is well known that hyperglycaemia due todiabetes mellitus leads to oxidative stress in the centralnervous system. Oxidative stress plays important role in thepathogenesis of neurodegenerative changes. In the presentstudy we investigated the possible neuroprotective effect ofetomidate against streptozotocin-induced (STZ-induced)hyperglycaemia in the rat brain and spinal cord. A total of40 rats were used in this study. Rats were divided into fourgroups: sham-control, diabetic, diabetic-etomidate treatedand vehicle for etomidate treatment group. Diabetes mellitus was induced by a single injection of streptozotocin(60 mg/kg body weight). Three days after streptoztocininjection, etomidate (2 mg/kg) was injected intraperitoneally for etomidate group and lipid emulsion (10%) forvehicle group was injected with corresponding amountintraperitoneally every day for 6 weeks. Six weeks afterstreptozotocin injection, seven rats from each group werekilled and brain, brain stem and cervical spinal cord wereremoved. The hippocampus, cortex, cerebellum, brain stemand spinal cord were dissected for the biochemical analysis

(the level of malondialdehyde [MDA], total nitrite, reducedglutathione [GSH], and xanthine oxidase [XO] activity).STZ-induced diabetes resulted in significantly elevation ofMDA, XO and nitrite levels in the hippocampus, cortex,cerebellum, brain stem and spinal cord of the rats(P < 0.05) while etomidate treatment provided significantly lower values (P < 0.05). This study demonstratedthat etomidate have neuroprotective effect on the neuronaltissue against the diabetic oxidative damage.Keywords Central nervous system Diabetes mellitus Etomidate Malondialdehide Oxidative damage StreptozotocinO. Ates (&) S. R. Cayli A. Kocak C. O. Cakir

Department of Neurosurgery, Turgut Ozal Medical Center,

Inonu University, School of Medicine, 44069 Malatya, Turkey

e-mail: [email protected]. YucelDepartment of Emergency Medicine, Inonu University, School

of Medicine, Malatya, TurkeyE. Altinoz Y. TurkozDepartment of Biochemistry, Inonu University, School of

Medicine, Malatya, TurkeyS. YologluDepartment of Biostatistics, Inonu University School of

Medicine, Malatya, TurkeyIntroductionDiabetes mellitus is a common epidemic endocrine disorder. Systemic complications due to hyperglycaemia is themain problem in diabetic patients. Hyperglycaemia provides more substrate for anaerobic glycoysis that leads tolactic acidosis [1]. Glycosylation, a nonenzymatic protein,is increased during diabetes and believed to contribute tothe long term complications of the diabetes...