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Received Aug 8, 2017; Accepted Nov 14, 2017
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1. Introduction
Analytical chemistry is a measurement science consisting of a set of powerful ideas and methods that are useful in all fields of science and medicine. It is applied throughout industry, medicine, and all the sciences [1].
Dosage has a primary role in the field of chemical analysis, allowing the user of a product or a substance quick and precise answers to any question related to its characterization.
Sucrose and other carbohydrates can be easily distinguished, either by taste or by means of easily developed physical and chemical reactions. The dosage of the latter can be achieved by several methods, either by physical, chemical, or biological methods using enzymes [2, 3].
Dosage by physical methods is used to dose sucrose in technical or purified sugar solutions, especially those which are marketed under the name of “liquid sugars”. These techniques will mainly be used for high-sucrose processed products [4]. The best known methods are polarimetry [5–7], refractometry [8, 9], and those using densimeter.
In spite of their high degree of accuracy, physical methods can only be used for the titration of pure sucrose solutions. If solutions are more complex, with the presence of reducing sugars, colorants, or flavor enhancers, chemical methods can be employed to determine sucrose content. The most widely used chemical methods are chromatography [10, 11] and reducing sugars, which are based on the reducing properties of free carbonyl group in reducing sugars, reacted with a copper solution in alkaline medium [12].
As for biological methods, their principle is extensively described in the specialized literature, especially in Bergmeyer’s work [13].
The objective of this paper is to develop a new method for the determination of sucrose by spectrophotometer. The influence of certain impurities, such as glucose and fructose, on the sucrose dosage, using this method, has been studied.
2. Materials and Methods
2.1. Reagents
The...