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J Mater Sci: Mater Med (2016) 27:140 DOI 10.1007/s10856-016-5756-8
BIOMATERIALS SYNTHESIS AND CHARACTERIZATION Original Research
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Web End = New synthesis method of HA/P(D,L)LA composites: study of bronectin adsorption and their effects in osteoblastic behavior for bone tissue engineering
Sabeha Yala1 Mahfoud Boustta2 Olivier Gallet3 Mathilde Hindi3Franck Carreiras3 Hamanou Benachour3 Djahida Sidane1 Hat Khireddine1
Received: 21 March 2016 / Accepted: 9 July 2016 / Published online: 17 August 2016 Springer Science+Business Media New York 2016
Abstract A novel synthetic method to synthesize hydro-xyapatite/poly (D,L) lactic acid biocomposite is presented in this study by mixing only the precursors hydroxyapatite and (D,L) LA monomer without adding neither solvent nor catalyst. Three compositions were successfully synthesized with the weight ratios of 1/1, 1/3, and 3/5 (hydroxyapatite/ (D,L) lactic acid), and the grafting efciency of poly (D,L) lactic acid on hydroxyapatite surface reaches up to 84 %. Scanning electron microscopy and Fourier transform infrared spectroscopy showed that the hydroxyapatite particles were successfully incorporated into the poly (D,L) lactic acid polymer and X ray diffraction analysis showed that hydroxyapatite preserved its crystallinity after poly (D,L) lactic acid grafting. Differential scanning calorimetry shows that Tg of hydroxyapatite/poly (D,L) lactic acid composite is less than Tg of pure poly (D,L) lactic acid, which facilitates the shaping of the composite obtained. The addition of poly (D,L) lactic acid improves the adsorption properties of hydroxyapatite for bronectin extracellular matrix protein. Furthermore, the presence of poly (D,L) lactic acid on hydroxyapatite surface coated with bronectin enhanced pre-osteoblast STRO-1 adhesion and cell spreading. These results show the promising potential of
hydroxyapatite/poly (D,L) lactic acid composite as a bone substitute material for orthopedic applications and bone tissue engineering.
1 Introduction
In the past decade, biodegradable nanocomposites of hydroxyapatite (HA) and biodegradable polymers have received a tremendous amount of interest as orthopedic implants, bone lling materials, and scaffolding materials for bone tissue engineering [16]. Due to its high bioactivity, biocompatibility, and osteoconductivity, apatite, especially HA called as bioceramic, is widely used as inorganic nanomaterial llers in polymeric matrixes for bone-like articial ceramic/polymer composites [712]. The application of HA is, nevertheless, limited by its brittleness, difculty in shaping, and slow biodegradation. Biodegradable polymers due to their excellent biocompatibility, biodegradability, processability, and approved use in medical devices by the US...