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Arch Virol (2008) 153:21452148 DOI 10.1007/s00705-008-0235-2
BRIEF REPORT
Nucleotide sequence and genome organization of a member of a new and distinct Caulimovirus species from dahlia
H. R. Pappu K. L. Druffel R. Miglino A. R. van Schadewijk
Received: 5 January 2008 / Accepted: 2 October 2008 / Published online: 31 October 2008 Springer-Verlag 2008
Abstract A distinct caulimovirus, associated with dahlia mosaic, was cloned and sequenced. The caulimovirus, tentatively designated as dahlia common mosaic virus (DCMV), had a double-stranded DNA genome of ca. 8 kb. The genome organization of DCMV was found to be typical of members of the genus Caulimovirus and consisted of six major open reading frames (ORFs), ORFs IVI, and one minor ORF, ORF VII. Sequence comparisons with the DNA genomes of two known caulimoviruses isolated from dahlia, Dahlia mosaic virus (DMV) and an endogenous caulimovirus, DMV-D10, showed that DCMV is a member of a distinct caulimovirus species, with sequence identities among various ORFs ranging from 25 to 80%.
Dahlia mosaic disease [1, 5, 6] is becoming an increasingly important disease of dahlia (Dahlia variabilis). Symptoms of dahlia mosaic include mosaic, chlorotic vein banding, systemic chlorosis, and leaf malformation. Dahlia mosaic virus (DMV), a distinct species in the genus Caulimovirus (family Caulimoviridae) has been described [6] and the physical map of the viral genome has been reported [21].
The genomic sequence of an isolate of this species was subsequently determined and is available in GenBank [3]. In addition to DMV, we recently characterized another caulimovirus that was found to be widely prevalent in dahlia in the US and elsewhere [1317, 19, 20]. Tentatively designated as DMV-D10, this caulimovirus was found to be signicantly divergent from DMV at nucleotide and amino acid sequence levels [14, 17]. Moreover, DMV-D10 was found to exist as an endogenous plant pararetorviral sequence (EPRV) in D. variabilis [18]. In addition to these two caulimoviruses, a partial genomic sequence representing the viral coat protein and reverse transcriptase genes of another caulimovirus has been recently reported [12] in dahlia samples originally collected in The Netherlands. As part of our ongoing studies on assessing the molecular variability of caulimoviruses associated with dahlia mosaic disease, we obtained the complete nucleotide sequence of a caulimovirus that was found in samples collected in the...