Optimization Concentration Control Cell Coombs (CCC) for Validity Tests on Crossmatching Examination

Abstract

Crossmatching is an examination that needs to be done before doing a blood transfusion to see whether the recipient’s blood is in accordance with the donor’s blood. Validity is done to find out the results obtained in all phases crossmatching tests correctly indicate compatible. Control Coombs Cell (CCC) is a control cell suspension made from group O Rh positive blood which is intentionally coated with an incomplete antibody. The aim is to determine the concentration and optimal time of CCC incubation so that it can be used for validity testing on crossmatching examination. The test used two-way ANOVA test showed significant results, there were differences in the degree of agglutination in variations in concentration and incubation time. The optimum results were not obtained at concentrations of 50 percents, 60 percents, 70 percents and the right incubation time of 15 minutes, 30 minutes, and 60 minutes. The results of agglutination were reduced at a concentration of 50 percents and an incubation time of 15 minutes, that coombs as controlling negative CCC test results and testing serum coombs. CCC as validating results so as not to cause false positive reactions or false negative results.