Optimization of Extraction conditions of the Polyphenols, Flavonoids and the Antioxidant activity of the plant Ammosperma cinereum (Brassicaceae) through the Response Surface Methodology (RSM)

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ABSTRACT:

This work focuses on the optimization of the extraction conditions, to extract the maximum polyphenols (TPC), flavonoids (TF) and for optimal antioxidant activity by the response method to (RSM) areas, from the aerial part of Ammosperma cinereum. Independent variables considered are: the methanol percentage (X₁: 60-100%), temperature (X₂: 20-60°C) and the extraction time (X₃: 20 -100 min). Results of ANOVA showed that the proportion of methanol, the temperature and duration of extraction affected TPC and TF, while the antioxidant activity was affected only by the methanol rate. The experimental data were well adjusted by polynomial models of the second order with R² = 0.986, 0.962 and 0.985 for TPC, TF and I% respectively (p <0.005). In addition, there's a close relationship between the predicted and experimental values. The optimum conditions for the highest performance of TPC (34.16 ± 0.24 mg EAG / g of extract), TF (29.37 ± 1.09 mg ER / g of extract) with 64.82% (± 0.26) of the antioxidant activity were obtained X₁ = 93.1%, X₂ = 36.9 ° C and for X₃ = 66.8 min.

KEYWORDS: Ammosperma cinereum, RSM, Box Behnken, TPC, TF, antioxidant activity.

(ProQuest: ... denotes formulae omitted.)

INTRODUCTION:

Recently, there has been a renewed interest in the plant secondary metabolites for their potential preventive effects on chronic diseases such as cardiovascular disease and cancer. Therefore, the isolation, identification and quantification of phytochemicals in food and assessment of their potential health benefits have been highlighted: However, in vitro and in vivo studies have shown that action of certain chemicals can be obtained by eating certain plants. Thus, extraction of the active ingredient is essential if it is to have a prophylactic or therapeutic value in humans1.

Phenolic compounds are common dietary phytochemicals found in fruits, vegetables and plant seeds2. The main feature of polyphenols is that they are very powerful antioxidants. Indeed, they are able to trap free radicals and activate other antioxidants in3,4 the body.

Ammosperma cinereum is a fairly gracile plant of the Brassicaceae family, it is localized only in southern Algeria. It is endemic to North Africa, annual and spontaneous5.

To date, no work has been done on the chemical composition or the optimization...