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Bulletin of Experimental Biology and Medicine, Vol. 146, No. 2, 2008 BIOPHYSICS AND BIOCHEMISTRY
203
Parameters of Nitrogen Metabolism during Insulin Hypoglycemia in Rats with Alloxan-Induced Diabetes
N. B. Medvedeva, P. L. Telushkin, and A. Yu. Stelmakh
Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 146, No. 8, pp. 168-170, August, 2008 Original article submitted July 3, 2007
Hypoglycemic coma caused by insulin injection to rats with alloxan-induced diabetes was accompanied by an increase in the concentrations of urea and uric acid and decrease in the content of free amino acids in blood plasma. Activities of glutamate dehydrogenase, AMP deaminase, glutaminase, ALT, and AST in the liver of experimental animals increased.
Key Words: insulin-induced hypoglycemia; alloxan-induced diabetes; liver; nitrogen metabolism
Diabetes mellitus is accompanied by an increase in the rate of protein degradation, gluconeogenesis from amino acids, production of ammonium, and production and excretion of urea [1]. Hypoglycemia is a common complication of therapy in patients with diabetes mellitus. This state also often leads to serious metabolic disorders [2,4,5]. Sharp variations in blood glucose level, changes in secretion of contrainsular hormones, and disturbed regulation can considerably modify metabolic response to hypoglycemia in diabetes mellitus [6].
This work was designed to study nitrogen metabolism during insulin-induced hypoglycemia in animals with experimental diabetes mellitus.
MATERIALS AND METHODS
Experiments were performed on male outbred albino rats weighing 200-240 g. The animals fed a standard diet. They were deprived of food, but had free access to water for 18-24 h before the experiment. The rats were divided into 4 groups. Group 1 consisted of intact animals (control). Group 2 animals were in hypoglycemic...