Content area
Full Text
Abstract
The present investigation evaluates the potential of aqueous seed extract of Abrus precatorius L. for the biosynthesis of silver nanoparticles (AgNPs). The structure of AgNPs was authenticated by the changes in colour as well as the UV-vis spectroscopy, which showed an absorbance maxima peak at 441 nm. The scanning electron microscope (SEM) investigation proved the particle shape as well as the X-ray diffraction (XRD) that validated the crystalline character of AgNPs. The AFM study also corroborated the surface morphology of manufactured AgNPs. Fourier Transform Infrared (FTIR) approved the presence of alcoholic, and the phenolic groups co-operated an imperative reduction role in the synthesis method. In vitro, the antioxidant action of both A. precatorius seed extract and AgNPs were scrutinized by DPPH assay. It illustrates the antibacterial activity against the gram negative bacteria Salmonella paratyphi as well as the Escherichia coli. Compared to other NPs, the AgNPs synthesized in this study were smaller in size that exhibited a higher level of antioxidant and antibacterial activity. From the consequences, it is proposed that green synthesized AgNPs could be employed successfully in future biomedical applications.
Keywords: Abrus Precatorius; Antibacterial Activity; DPPH Radical Scavenging; Silver Nanoparticles; X-ray Diffraction.
INTRODUCTION
In the 21st century, nanotechnology is expected to be the foundation of many biotechnology innovations and is also considered to be the upcoming industrial revolution. Nanomaterials are called a "wonder of modern medicine" and extracted much attention over the past few decades [1]. Nanomaterials are of great significance because of their superior physicochemical and biological characteristics over their bulkiness phase. The size of these nanostructured materials (1-100 nm) recommends a higher surface to volume ratio which leads to high surface reactivity [2]. This distinct property allowed them to be utilized in vast applications in many fields ranging from material science to biotechnology [3]. The metals such as gold, silver and copper have been employed extensively for the fusion of stable dispersion of nanoparticles, which are highly competent in the fields such as biological labeling, photonics and Surface Enhanced Raman Scattering (SERS) detection [4-6].
Researchers are discovering green synthesis of Ag nanoparticles to decrease the exercise of toxic and hazardous reactants and formation of by-products figured during the reaction. The green synthesis of the AgNPs being used in medicinal...