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Eur J Nutr (2012) 51:961973 DOI 10.1007/s00394-011-0274-4
ORIGINAL CONTRIBUTION
Purple corn anthocyanins retard diabetes-associated glomerulosclerosis in mesangial cells and db/db mice
Jing Li Min-Kyung Kang Jin-Kyu Kim
Jung-Lye Kim Sang-Wook Kang
Soon Sung Lim Young-Hee Kang
Received: 26 August 2011 / Accepted: 1 November 2011 / Published online: 20 November 2011 Springer-Verlag 2011
AbstractPurpose Diabetic glomerulosclerosis is the hardening of the renal glomeruli that can lead to kidney failure. In the early stage of glomerulosclerosis occur renal mesangial expansion and renal ltration dysfunction. Purple corn has been classied as a functional food and is rich in anthocyanins exerting potential disease-preventive activities. The in vitro study using human renal mesangial cells examined that anthocyanin-rich purple corn butanol fraction (PCB) can attenuate high glucose (HG)-promoted mesangial cell proliferation and matrix accumulation. Methods Cells were cultured for 3 days in media containing 33 mM glucose in the presence of 120 lg/mL
PCB. In the in vivo animal study, db/db mice were treated with 10 mg/kg anthocyanin-rich polyphenolic extracts of purple corn (PCE) for 8 weeks.
Results HG enhanced mesangial production of the brosis biomarkers of collagen IV and connective tissue growth factor (CTGF), which was markedly attenuated by adding PCB. Such mesangial brosis entailed interleukin-8 activation via eliciting Tyk2-STAT signaling pathway. PCB dampened HG-promoted mesangial hyperplasia that
appeared to be attributed to increased expression of platelet-derived growth factor. The 8-week administration of PCE lowered plasma glucose level of db/db mice and ameliorated severe albuminuria. Moreover, PCE lessened collagen ber accumulation in kidney glomeruli and CTGF expression via retarding TGF-b signaling. Protein expressions of nephrin and podocin, key proteins for ltration barrier function of the glomerular capillary wall, were repressed by treating mice with PCE.
Conclusion Purple corn may be a potent therapeutic agent for the treatment for diabetes-associated glomerulo-sclerosis accompanying proteinuria and kidney ltration dysfunction.
Keywords db/db mice Glomerulosclerosis High
glucose Mesangial cells Purple corn anthocyanin
Renal brosis
AbbreviationsCTGF Connective tissue growth factorDN Diabetic nephropathyECM Extracellular matrixHG High glucoseHRMC Human renal mesangial cellsIL InterleukinMMP Matrix metalloproteinasesMT1-MMP Membrane type-1 matrix metalloproteinase PCB Anthocyanin-rich purple corn butanol extractPCE Anthocyanin-rich polyphenolic extracts of purple cornPDGF Platelet-derived growth factorTGF Transforming growth factorTIMP Tissue inhibitor of metalloproteinases
Jing Li and Min-Kyung Kang contributed equally to this work.
J. Li M.-K. Kang J.-L. Kim...