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Abstract: The present study was undertaken with an objective to find out the antibacterial activity of Ficus Recemosa Linn. Leaves against Actinomycets viscosus .The hydro alcoholic extract of Ficus Recemosa Linn. Was found effective against Actinomyces vicosus (MTCC 7345). The minimum inhibitory concentration (MIC) was determined using Broth dilution technique and found to be 0.08mg/ml. The zone of inhibition was measured using Cup plate diffusion technique.
Key Words: Antibacterial activity, Ficus Recemosa, Actinomyces viscosus, leaves.
Introduction: Ficus Recemosa Linn is a large dedicious tree distributed throughout India particularly in evergreen forests and moist localities11.Root bark, leaves fruit and galls are part of tree used for therapeutic activity. Bark, leaves and unripe fruit are carminative, astringent, stomachic and vermicide. As mentioned in the Ayurvedic Nighanthus that the infusion of the bark, fruit and leaves is cooling, sweet and astringent2. The bark chemically constitutes of gluanol acetate, beta-sitosterol, leucocynedin and leaf chemically contain beta-amyrin, beta-sitosterol and tannin. Fruit chemically contain lupeol-OAc, glucose, sterol, and gluanol-OAc3-5.
Actinomyces viscosus belongs to group of actinomycets. It is gram positive, aerobic, non sporing, rod shaped bacteria. The mouths contain a wide variety of bacteria, but only a few specific species of bacteria are believed to dental cares. For root caries the most closely associated bacteria frequently identified are Actinomyces viscosus, Nocardia spp and streptococcus mutans. Actinomyces viscosus is frequently encountered in high proportion of smooth tooth surface and gingiva6-9.
Antibacterial Tests are indicated for those organisms contributing to the infectious process whose susceptibility can not be predicted from knowledge of their identity. The Antibacterial activity has been done by two methods.
Dilution method is used to determine the minimal concentration usually expressed in units or microorganism per ml of an antimicrobial agent required to inhibit or kill a microorganism. Diffusion method is used to test the bacterial susceptibility to antimicrobial agent and may be measured in-vitro by using the...