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Abstract

Issue Title: Quantification of a peptide standard using intrinsic fluorescence/In situ monitoring, separation, and characterization of gold nanorods/Quantification of Legionella pneumophila in surface water/Printing metal-inks for LA-ICP-MS bioimaging standardization

The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.

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Details

Title
Removal of Polysorbate 80 by complexation prior to LC-MS analysis
Author
Jäpelt, Kristina B; Johnsen, Lea Giørtz; Christensen, Jan H
Pages
2303-2307
Publication year
2016
Publication date
Mar 2016
Publisher
Springer Nature B.V.
ISSN
16182642
e-ISSN
16182650
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1781217240
Copyright
Springer-Verlag Berlin Heidelberg 2016