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ABSTRACT:
A reverse phase high performance liquid chromatography (RP-HPLC) has been developed and validated as a stability indicating assay of Ibuprofen (IBP) and Methocarbamol (MCB). The degradants formed were well separated from the main peaks. The separation was achieved by isocratic elution on Inertsil ODS-3V Column (150 mm x 4.6mm x 5 µm) and 0.1M Phosphate buffer pH 3.5: methanol: acetonitrile and tetrahydrofuran (20:25:55:0.3) used as a mobile phase at a flow rate of 1ml/min. The detection was carried out at 220nm. Retention time of Ibuprofen and Methocarbamol were found to be 8.6 and 3.7 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Ibuprofen and Methocarbamol were in the range of 25%-175% concentration of standard preparation. The mean recoveries obtained for Ibuprofen and Methocarbamol were found to be 100.61% and 99.23% respectively. Developed RP-HPLC method was found to be accurate, precise, selective and rapid for simultaneous estimation of Ibuprofen and Methocarbamol in tablets.
KEYWORDS: RP-HPLC; Ibuprofen; Methocarbamol and Recovery
(ProQuest: ... denotes formulae omitted.)
INTRODUCTION:
Ibuprofen1 (IBP) is chemically (RS)-2-(4-(2-methylpropyl) phenyl) propionic acid, it is come under category of Nonsteroidal anti-inflammatory drug (NSAID). Ibuprofen is an inhibitor of the enzyme cyclooxygenase (COX), which converts arachidonic acid to prostaglandin H2 (PGH2). PGH2, in turn, is converted by other enzymes to several other prostaglandins (which are mediators of pain, inflammation, and fever) and to thromboxane A2 (which stimulates platelet aggregation, leading to the formation of blood clots).
Methocarbamol1 (MCB) is chemically 2-hydroxy-3-(2- methoxyphenoxy) propyl carbamate, it is a centrally acting skeletal muscle relaxant which reduces the skeletal muscle tone by a selective action on the cerebral spinal axis, without altering consciousness.
Basu D et al2. have suggested a method using high performance liquid chromatography for the determination of ibuprofen and paracetamol in tablets. Zarapkar S.S. et al3.have reported, Reverse phase HPLC determination of ibuprofen, paracetamol and methocarbamol in tablets. Erk N et al4.published a method of Simultaneous determination of paracetamol and methocarbamol in tablets by ratio spectra derivative spectrophotometry.
Literature survey reveals that no stability indicative analytic method for the estimation of Ibuprofen and Methocarbamol combination of drugs. Hence, there is a need to develop and validate as an stability indicating analytical method for the estimation of Ibuprofen and Methocarbamol.