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Abstract
Urease plays a crucial role in the survival and pathogenesis ofHelicobacter pylori(H. pylori), and antibody neutralizing the urease activity may be implicated for the protection againstH. pyloriinfection. Previously, a neutralizing monoclonal antibody (MAb) 6E6 against UreB ofH. pyloriwas developed. In this work, we try to identify the B-cell epitope recognized by neutralizing MAb 6E6. Following screening a series of truncated proteins of UreB, an epitope was primarily localized in the aa 200-230 of UreB. Subsequently, we screened the overlapping synthetic peptides covering the aa 200-230 and identified a novel B-cell epitope (U211-225, IEAGAIGFKIHEDWG) that was recognized by specific MAb 6E6. The newly identified epitope may help understanding of the protective immunity againstH. pyloriand be implicated for vaccine development.