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© 2020 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Several signaling exchanges between oocytes and pre-granulosa cells are also implicated in such processes, such as Notch, KIT proto-oncogene, receptor tyrosine kinase ligand (KITL)/KIT system, neurotrophins, transforming growth factor-beta (TGF-beta) and its family members growth differentiation factor 9 (GDF9), BMP15, Activin A, inhibin, follistatin, and anti-Mullerian hormone, as well estrogens, progesterone, and finally follicle-stimulating hormone (FSH) [1,15]. Despite these results and previous transcriptome analyses performed in rat [26,27] and mouse fetal and early postnatal ovaries [28,29], the precise molecular mechanisms underlying oocyte survival/death, granulosa cell differentiation, and the crosstalk among them during PF assembly are still incomplete. [...]all the latter studies were performed on the entire ovary or follicles so that the contribution of each cell type to the transcriptomes was not distinguishable. According to uniform manifold approximation and projection (UMAP), the cell populations at 3 developmental stages were clustered (Fig 1B), and 7 cell types were identified, and according to their distinct transcriptomic signatures (S1 Table), they could be subdivided as follows: germ cells, granulosa cells, stromal cells, erythrocytes, immune cells, endothelial cells and epithelial cells (Fig 1C and 1D and S1D Fig). According to these analyses, a genetic dynamic model, including pre-, early- and late-follicle formation stages during germ cell development was drawn (Fig 2C and 2D).

Details

Title
Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice
Author
Jun-Jie, Wang; Ge, Wei  VIAFID ORCID Logo  ; Qiu-Yue Zhai; Jing-Cai, Liu  VIAFID ORCID Logo  ; Xiao-Wen, Sun; Wen-Xiang, Liu; Li, Lan; Chu-Zhao, Lei; Dyce, Paul W  VIAFID ORCID Logo  ; De Felici, Massimo  VIAFID ORCID Logo  ; Shen, Wei  VIAFID ORCID Logo 
First page
e3001025
Section
Methods and Resources
Publication year
2020
Publication date
Dec 2020
Publisher
Public Library of Science
ISSN
15449173
e-ISSN
15457885
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2479139902
Copyright
© 2020 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.