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Abstract The research explored the effects of carbon, nitrogen and inorganic salt nutritional factors on Lactarius deliciosus hyphae as per solid culture. The optimal recipe of culture medium was selected by observing hypha density and computing the growth index, which is the most suitable for hypha growth. The results indicated that glucose and peptone were optimal carbon and nitrogen for Lactarius deliciosus; MgSO^sub 4^, CuSO^sub 4^ and VB^sub 2^ promoted hypha growth; FeSO^sub 4^ and CoCl^sub 2^ had inhibition effects. The orthogonal test concluded the optimal recipe contained glucose of 20 g/L, peptone of 1 g/L, KH^sub 2^PO^sub 4^ of 0.3 g/L, MgSO^sub 4^ of 0.3 g/L, VB^sub 2^ of 2 mg/L, and agar of 20 g/L, with original pH value adopted. After optimization, growth index of hyphae cultured at 28 °C was 4 055.9.
Key words Lactarius deliciosus; Optimal medium; Growth index of hypha
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actarius deliciosus, commonly known as red pine mushroom and saffron milk cap, is of the large milk-cap genus Lactarius in the order Russulales. It is a delicious wild edible mushroom111. Furthermore, it performs well in controlling tumour and the produced lactaroviolin has an antibacterial action12-31. Besides, the sporocarps would produce sphingolipids and lectins141, of which polysaccharides plays the role of antineoplastic activity and immunoregulatory activity15-61. Because Lactarius deliciosus keeps symbiotic relationship with plants, it has a special living condition, nutrition-absorption way and fruitbody differentiation condition. Therefore, it is difficult for domestication and the cultivation at large-scale is not available at home and abroad yet17-81. On the other hand, researches are few on recipe of solid medium of Lactarius deliciosus and the cultivation conditions. The research selected the optimal cultivation medium recipe and condition as per solid culture of Lactarius deliciosus based on nutrient factors and cultivation conditions in order to further develop Lactarius deliciosus and provide theoretical references for artificial cultivation of Lactarius deliciosus.
Materials and Methods
Materials
Test strains Lactarius deliciosus was provided by Yichun Vocational Technical College.
Culture media Medium I contained potato of 200 g, glucose of 20 g, KH2P04 of 1 g, MgS04 of 0.5 g, agar of 20 g, and distilled water of 1 000 ml, with original pH value adopted.
Medium II contained glucose of 20...