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J Assist Reprod Genet (2012) 29:310 DOI 10.1007/s10815-011-9644-3
GAMETE BIOLOGY
Sperm transcriptome profiling in oligozoospermia
Debbie Montjean & Pierre De La Grange &
David Gentien & Audrey Rapinat & Stphanie Belloc &
Paul Cohen-Bacrie & Yves Menezo & Moncef Benkhalifa
Received: 16 July 2011 /Accepted: 28 September 2011 /Published online: 12 October 2011 # Springer Science+Business Media, LLC 2011
AbstractPurpose Investigate in what extent sperm transcriptome of infertile men is different from that of fertile individuals. Methods Semen samples were collected for determination of sperm parameters as well as for RNA isolation. Gene expression profile was investigated in spermatozoa of 8 infertile and 3 fertile men by microarray analysis using the Affymetrix Chip HG-U133 Plus 2.0.
Capsule Sperm transcriptome is altered in oligozoospermia.
This work has been presented at the 27th Annual Meeting of ESHRE, Rome 2010.
We analyzed and compared the whole transcriptome profile of oligoasthenoteratozoospermic sperm patients to normal fertile men to understand gene expression deregulation associated with oligoasthenoteratozoospermia.
D. Montjean : M. Benkhalifa Advanced Technology Laboratory, ZA de lAgiot 4 rue Louis Lormand, 78320 La Verrire Paris, France
D. Montjean (*) : S. Belloc : P. Cohen-Bacrie : Y. Menezo :
M. BenkhalifaLaboratoire dEylau, Unilabs,Paris, Francee-mail: [email protected]
P. De La Grange GenoSplice Technology, Paris, France
D. Gentien : A. RapinatPlatform of Molecular Biology Facilities, Translational Research Department, Research center, Institut Curie,Paris, France
Result(s) We observed up to 33-fold reduction expression of genes involved in spermatogenesis and sperm motility. Furthermore, there is an important decrease in expression of genes involved in DNA repair as well as oxidative stress regulation. In this study, we also show a striking drop in expression of histone modification genes.
Conclusion(s) We found that transcription profile in germ cells of men with idiopathic infertility is different from that of fertile individuals. Interestingly, about 15% of the regulated genes (Eddy Rev Reprod 4:2330, 1999) play a role in spermatogenesis.
Keywords Sperm . Transcripts .
Oligoasthenoteratozoospermia . Male infertility. Microarray
Introduction
Approximately 15% of the couples worldwide have fertility problems with a male factor found in 45% of the cases. Multiple factors are known to impair spermatogenesis: exposure to environmental disruptors, genetics, testis pathologies, lifestyle [1, 2].However, about two-thirds of the cases of non-obstructive spermatogenic defects with apparently normal semen quality...