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Stem Cell Rev and Rep (2012) 8:184194 DOI 10.1007/s12015-011-9272-4
Stem Cells, Including a Population of Very Small Embryonic-Like Stem Cells, are Mobilized Into Peripheral Blood in Patients After Skin Burn Injury
Justyna Drukaa & Edyta Paczkowska & Magda Kucia & Elbieta Myska &
Andrzej Krajewski & Bogusaw Machaliski & Zbigniew Madeja &
Mariusz Z. Ratajczak
Published online: 15 May 2011# Springer Science+Business Media, LLC 2011
AbstractBackground Developmentally early cells, including hematopoietic stem progenitor cells (HSPCs), as well as very small embryonic-like stem cells (VSELs), are mobilized into peripheral blood (PB) in response to tissue and organ injury (e.g., heart infarct or stroke).
Objective We seek to determine whether these cells are also mobilized into PB in patients with skin burn injuries.
Methods Forty-four (44) patients (3357 years of age) with total body surface burn area of 3060%, as well as 23 healthy control subjects, were recruited and PB samples were harvested during the first 24 hours, day +2, and day +5 after burn injury and compared to normal controls. The circulating human CD34+CD133+ cells
enriched for HSPCs, as well as small CXCR4+CD34+CD133+ subsets of LinCD45 cells that correspond to the population of VSELs, were counted by FACS and evaluated by direct immunofluorescence staining for pluripotency markers (Oct-4, Nanog, and SSEA-4). In parallel, we also measured by ELISA the serum concentration of factors that regulate stem cell trafficking, such as SDF-1, VEGF, and HGF.
Results Our data indicate that skin burn injury mobilizes cells expressing stem cell-associated markers, such as CD133, CD34, and CXCR4, into PB. More importantly, we found an increase in the number of circulating primitive, small Oct-4+Nanog+SSEA-4+CXCR4+linCD45 VSELs. All these changes were accompanied by increased serum concentrations of SDF-1 and VEGF.
J. Drukaa (*)
Laboratory of Cell &Tissue Engineering,Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology,Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7,30387 Cracow, Polande-mail: [email protected]
Z. MadejaLaboratory of Cell & Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7,30387 Cracow, Poland
E. Paczkowska : B. MachaliskiDepartment of Physiopathology, Pomeranian Medical University, Szczecin, Poland
M. Kucia : M. Z. Ratajczak (*)
Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville,580 S Preston St, Baxter II, Rm 119E,Louisville, KY 40202, USAe-mail: [email protected]