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Glycoconj J (2013) 30:497510 DOI 10.1007/s10719-012-9450-x
Structural remodeling of proteoglycans upon retinoic acid-induced differentiation of NCCIT cells
Leyla Gasimli & Hope E. Stansfield & Alison V. Nairn &
Haiying Liu & Janet L. Paluh & Bo Yang &
Jonathan S. Dordick & Kelley W. Moremen &
Robert J. Linhardt
Received: 19 July 2012 /Revised: 8 September 2012 /Accepted: 10 September 2012 /Published online: 2 October 2012 # Springer Science+Business Media, LLC 2012
Abstract Pluripotent and multipotent cells become increasingly lineage restricted through differentiation. Alterations to the cellular proteoglycan composition and structure should accompany these changes to influence cell proliferation, delineation of tissues and acquisition of cell migration capabilities. Retinoic acid plays an important role in pre-patterning of the early embryo. Retinoic acid can be used in vitro to induce differentiation, causing pluripotent and multipotent cells to become increasingly lineage restricted. We examined
retinoic acid-induced changes in the cellular proteoglycan composition of the well-characterized teratocarcinoma line NCCIT. Our analysis revealed changes in the abundance of transcripts for genes encoding core proteins, enzymes that are responsible for early and late linkage region biosynthesis, as well as enzymes for GAG chain extension and modification. Transcript levels for genes encoding core proteins used as backbones for polysaccharide synthesis revealed highly significant increases in expression of lumican and decorin, 1,500-
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s10719-012-9450-x
Web End =10.1007/s10719-012-9450-x ) contains supplementary material, which is available to authorized users.
L. Gasimli : J. S. Dordick : R. J. LinhardtDepartment of Biology, Center for Biotechnologyand Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
H. Liu : B. Yang : R. J. LinhardtDepartment of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute,Troy, NY 12180, USA
J. S. Dordick : R. J. LinhardtDepartment of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute,Troy, NY 12180, USA
J. S. Dordick : R. J. Linhardt (*)
Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street,Troy, NY 12180, USAe-mail: [email protected]
H. E. StansfieldDepartment of Orthopedics, Center for Muscoloskeletal Research, University of Rochester Medical Center,Rochester, NY 14642, USA
A. V. Nairn : K. W. MoremenComplex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
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