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Web End = Cell Biochem Biophys (2015) 71:13791385
DOI 10.1007/s12013-014-0360-3
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Web End = Synergistic Anti-Cancer Effects of Icariin and Temozolomide in Glioblastoma
Lijuan Yang Yuexun Wang Hua Guo
Meiling Guo
Published online: 11 November 2014 Springer Science+Business Media New York 2014
Abstract Glioblastoma is an aggressive malignancy, which is associated with poor prognosis. Temozolomide (TMZ) has been showed to be an effective chemotherapeutic agent for glioblastoma treatment; however, the response rate is not satisfactory. Icariin is a natural compound with anti-cancer activity against a variety of cancers. This study is designed to determine whether icariin could potentiate the antitumor activity of TMZ in glioblastoma. Cell proliferation and apoptosis were measured using MTT assay and ow cytometry, respectively. Expression of apoptosis and proliferation-related molecules was detected by Western blotting while NF-jB activity was detected by
ELISA. Icariin dose-dependently inhibited proliferation and induced apoptosis in tested glioblastoma cell lines. Icariin enhanced the anti-tumor activity of TMZ in vitro. The anti-tumor activity of icariin and the enhanced anti-tumor activity of TMZ by icariin correlated with suppression of NF-jB activity. Our results showed that icariin exhibited anti-tumor activity and potentiated the anti-tumor activity of TMZ in glioblastoma, at least in part, by inhibiting NF-jB activity. Although more studies including clinical trials are needed, this study provides insight for using icariin as a chemosensitizing agent in clinic settings.
Keywords GBM TMZ Icariin AMPK
Introduction
Glioblastoma multiforme (GBM), accounting for approximately 60 % of all brain tumors, is the most common type of primary brain tumors in adults [1, 2]. GBMs are one of the most lethal, highly invasive, and least effectively treated solid tumors. Despite aggressive therapy regimen including maximal surgical resection, combined radiation/ chemotherapy, the recurrence of GBM is still quite common [3, 4]. Due to the ability to inltrate diffusely into the normal brain parenchyma, the prognosis of patients with the highest grade malignant glioma, GBM, remains poor with a median survival of at 1215 months and a 5-year survival rate of 2 % [4]. Therefore, improvement of treatment options for patients...