Content area
Full Text
http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = Antonie van Leeuwenhoek (2015) 108:14571468 DOI 10.1007/s10482-015-0601-5
http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = ORIGINAL PAPER
http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10482-015-0601-5&domain=pdf
Web End = Taxonomic characterisation of Proteus terrae sp. nov., a N2O-producing, nitrate-ammonifying soil bacterium
Undine Behrendt . Jrgen Augustin . Cathrin Sprer . Jrg Gelbrecht . Peter Schumann . Andreas Ulrich
Received: 25 June 2015 / Accepted: 28 September 2015 / Published online: 5 October 2015 Springer International Publishing Switzerland 2015
Abstract In the context of studying the inuence of N-fertilization on N2 and N2O ux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was inuenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus,
showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNADNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687T =DSM 29910T =LMG 28659T) is proposed.
Keywords Proteus terrae sp. nov. Phylogenetic
characterization Phenotypic characterisation
Dissimilatory nitrate reduction to ammonium Nitrous
oxide production C/N ratio Fen peat soil
Introduction
The genus Proteus of the family Enterobacteriaceae, established by Hauser early in the history of micro-biology, has been subjected to an eventful taxonomic
The EMBL accession number for the 16S rRNA gene sequences of the type strain Proteus terrae N5/687T (DSM 29910T = LMG 28659T) and Proteus penneri DSM 4544T are LN680103 and LN809884, respectively. The rpoB gene sequences of Proteus terrae N5/687T and Proteus hauseri DSM 14437T are LN809885 and LN809886, respectively
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s10482-015-0601-5
Web End =10.1007/s10482-015-0601-5 ) contains...