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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Soybean is the primary source of vegetable protein and is used for various purposes, mainly to feed animals. This crop can have diverse seed coat colors, varying from yellow, black, brown, and green to bicolor. Black seed coat cultivars have already been assigned as favorable for both seed and grain production. Thus, this work aimed to identify genes associated with soybean seed quality by comparing the transcriptomes of soybean seeds with contrasting seed coat colors. The results from RNA-seq analyses were validated with real-time PCR using the cultivar BRS 715A (black seed coat) and the cultivars BRS 413 RR and DM 6563 IPRO (yellow seed coat). We found 318 genes differentially expressed in all cultivars (freshly harvested seeds and seeds stored in cold chamber). From the in silico analysis of the transcriptomes, the following genes were selected and validated with RT-qPCR: ACS1, ACSF3, CYP90A1, CYP710A1, HCT, CBL, and SAHH. These genes are genes induced in the black seed coat cultivar and are part of pathways responsible for ethylene, lipid, brassinosteroid, lignin, and sulfur amino acid biosynthesis. The BRSMG 715A gene has almost 4times more lignin than the yellow seed coat cultivars. These attributes are related to the BRSMG 715A cultivar’s higher seed quality, which translates to more longevity and resistance to moisture and mechanical damage. Future silencing studies may evaluate the knockout of these genes to better understand the biology of soybean seeds with black seed coat.

Details

Title
Transcriptional Profile of Soybean Seeds with Contrasting Seed Coat Color
Author
Kafer, João M 1 ; Molinari, Mayla D C 2 ; Henning, Fernando A 3 ; Koltun, Alessandra 4   VIAFID ORCID Logo  ; Marques, Viviani V 2   VIAFID ORCID Logo  ; Marin, Silvana R R 3 ; Nepomuceno, Alexandre L 3 ; Mertz-Henning, Liliane M 3   VIAFID ORCID Logo 

 Biotechnology Department, Londrina State University, Londrina 86057-970, PR, Brazil 
 Arthur Bernardes Foundation, Embrapa Soja, Londrina 86085-981, PR, Brazil; [email protected] (M.D.C.M.); [email protected] (V.V.M.) 
 Embrapa Soja, Londrina 86085-981, PR, Brazil; [email protected] (F.A.H.); [email protected] (S.R.R.M.); [email protected] (A.L.N.) 
 Agronomy Department, State University of Maringá, Maringá 87020-900, PR, Brazil; [email protected] 
First page
1555
Publication year
2023
Publication date
2023
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2799652009
Copyright
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.