ARTICLES
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
Claudia Lamarca Vitral; Ana Maria Coimbra Gaspar; Clara Fumiko Tachibana Yoshida
Adress to correspondence
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ABSTRACT
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.
Keywords: Enzyme immunoassay. Anti-HAV. Hepatitis A.
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RESUMO
Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa.
Palavras-chave: Enzyme immmunoassay. Anti-HAV. Hepatitis A.
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REFERENCES
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Adress to correspondence:
Dra. Claudia Lamarca Vitral.
Depto. of Virology/FIOCRUZ.
Av. Brasil 4365
21040
Rio de Janeiro, RJ, Brazil.
Recebido para publicação em 16/01/91.
Departamento of Virology, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
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Copyright Sociedade Brasileira de Medicina Tropical Apr/Jun 1991
Abstract
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer