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Bulletin of Experimental Biology and Medicine, Vol. 146, No. 1, 2008 CELL TECHNOLOGIES IN BIOLOGY AND MEDICINE
153
The Use of Bioactive Wound Dressing, Stimulating Epithelial Regeneration of IIIa-Degree Burn Wounds
A. S. Ermolov, S. V. Smirnov, V. B. Khvatov, L. P. Istranov,
O. I. Koniushko, E. G. Kolokolchikova, M. V. Sychevsky,
and V. S. Bocharova
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 166-170, August, 2008 Original article submitted December 29, 2007
Clinical and morphological comparison of wound healing after transplantation of living cultured allofibroblast on days 1-2 after the injury, collagen-1-based dressing with PDGF-BB, and traditional dressing with levomecol ointment showed that bioactive dressing accelerated wound epithelialization (5-7 days vs. 20-22 days with gauze dressing); the incidence of suppurative complications decreased, no crust formed, and epithelialization was not associated with the formation of a hypertrophic cicatrix. Biological dressing based on living cultured allofibroblasts and collagen-1 with PDGF-BB exhibited equal stimulatory effects on burn wound healing.
Key Words: IIIa-degree burn wound; biological dressing; allofibroblasts; collagen-1; PDGF-BB
About 500,000 patients have burn injuries in Russia annually, which necessitates search for new approaches to the treatment of burn wounds. Data on the role of stem cells (SC), cell-matrix interactions, involvement of growth factors and cytokines in reparative processes have been accumulated over the recent decade [8,10,14,16]. However, these factors do not receive due attention in local treatment of burn wounds.
According to modern concepts, two SC types are distinguished: cells of one type in the keratinocyte basal layer are responsible for the maintenance of normal epidermis structure, cells of other type in skin derivatives (hair follicles, sebaceous and sweat glands) actively repopulate the epidermis only in case of its damage [10].
IIIa-degree burn is a sort of a borderline between skin capacity to spontaneous healing and restoration of the skin integument by skin auto-transplantation in IIIb-IV-degree burns. We should
like to emphasize that skin derivative SC population provides spontaneous epithelialization of IIIa-degree burns.
The exceptional role of fibroblasts in skin wound healing, specifically, their migration to the focus of injury, is well known. It is hypothesized that fibro-blasts migrate into the wound from intact derma and bone marrow no earlier than 5-7 days after the injury [9,15]. One of important factors initiating...