Abstract

The purpose of this study was to determine whether a proprietary xeno-free synthetic culture surface could be used to aid in the production and subsequent retinal-specific differentiation of clinical-grade induced pluripotent stem cells (iPSCs). It was found that Synthemax cell culture surfaces provide an ideal surface for the xeno-free production, culture, and differentiation of adult somatic cell-derived iPSCs. These findings demonstrate the potential utility of these surfaces for the production of clinical-grade retinal neurons for transplantation and induction of retinal regeneration.

Details

Title
Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation
Author
Tucker, Budd A 1 ; Anfinson, Kristin R 1 ; Mullins, Robert F 1 ; Stone, Edwin M 2 ; Young, Michael J 3 

 Institute for Vision Research, Carver College of Medicine 
 Institute for Vision Research, Carver College of Medicine; Howard Hughes Medical Institute, Department of Ophthalmology, University of Iowa, Iowa City, Iowa, USA 
 Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, USA 
Pages
16-24
Section
Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells
Publication year
2013
Publication date
Jan 2013
Publisher
Oxford University Press
ISSN
21576564
e-ISSN
21576580
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2289628135
Copyright
© 2013. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.