Certains cas d'infertilite sont caracterises par un faible niveau de P34H: Une proteine epididymaire humaine situee sur l'acrosome du spermatozoide

Abstract

La liaison du spermatozoide a la zone pellucide est une etape critique lors de la fecondation. Les molecules a la surface des gametes males et femelles interagissent entre elles afin de produire la liaison et eventuellement la penetration. Au niveau de la membrane plasmique du spermatozoide, des proteines sont ajoutees ou modifiees au cours de la maturation epididymaire. Notre laboratoire a prealablement identifie une glycoproteine membranaire qui s'associe a la region acrosomique du spermatozoide de hamster lors du transit epididymaire. Cette proteine, la P26h, joue un role essentiel dans la liaison du spermatozoide avec la zone pellucide. L'homologue de la P26h, la P34H, a ete caracterisee et identifiee chez l'humain comme etant un marqueur de la fertilite masculine.

Notre etude a comme objectif d'evaluer le niveau de la P34H presente sur les spermatozoides d'hommes consultant pour differentes causes d'infertilite. L'anticorps polyclonal anti-P26h qui reconnai t plusieurs proteines spermatiques de differentes especes, nous a permis de reveler la presence de P34H par des analyses Western blots.

La P34H est un marqueur biochimique qui pourrait s'averer utile dans le diagnostic d'infertilite-masculine.

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Binding of sperm to the zona pellucida is a critical step during fertilization. Molecules on the surface of male and female gametes interact with each other to produce bonding and eventually penetration. At the level of the sperm plasma membrane, proteins are added or modified during epididymal maturation. Our laboratory has previously identified a membrane glycoprotein which associates with the acrosomal region of hamster spermatozoa during epididymal transit. This protein, P26h, plays an essential role in the connection of the sperm to the zona pellucida. The homolog of P26h, P34H, has been characterized and identified in humans as being a marker of male fertility.

Our study aims to evaluate the level of P34H present in the spermatozoa of men consulting for different causes of infertility. The anti-P26h polyclonal antibody, which recognizes several sperm proteins from different species, allowed us to reveal the presence of P34H by Western blot analyses.

P34H is a biochemical marker that could prove useful in the diagnosis of male infertility.