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Abstract: The tissue-cultured shoot primordia have distinct characteristics of high stability of chromosome number, high ability of regeneration and rapid proliferation. Rapid propagation of Chrysanthemum morifolium Ramat. "Prince de Monaco" and "Romica" was achieved through tissue culture technique. The procedure involved aseptic culture of shoot tips followed by rapid shoot multiplication, rooting and finally establishment of plantlets in soil. The agar solidified MS medium containing 2 mg/l BAP and 0,002 mg/l NAA was optimum for rapid mass production of plantlets. These can be rooted on MS medium in the absence of growth regulators. The indirect micropropagation of Chrysanthemum morifolium Ramat. was achieved through callus cultures obtained from single-node stem segments excised from other two cultivars of Chrysanthemum morifolium Ramat.: "Escorte" and "La Cagouille", belonging to the collection of Botanical Garden from Iasi.
Key words: Chrysanthemum, micropropagation, shoot tips, callus
Introduction
The modern approaches of plant propagation based on cell and tissue culture techniques are able to increase the efficiency of breeding processes [1], [2]. They offer opportunities for rapid clonal propagation of some unique, superior genotypes[3], [4]. The unconventional techniques permit the multiplication and maintenance of these genotypes.
Material and methods
Shoot apices, detached with a small portion of cotyledonary and hypocotyle tissues were sterilized with calcium hypochlorite 3 % and cultured " in vitro" in 12 hrs light and 8 hrs darkness dayly at 24° C on MS medium with 2mg/l BAP and 0,002 mg/l NAA. Shoots of 2-3 cm, elongated "in vitro" were rooted on the MS medium both in the absence of growth regulators and by adding low concentration of BAP (2mg/l).
Explants consisting in single-node stem segments were cultured on MS medium...